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Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming

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Contact Information	Please send any questions regarding this item to libref@uno.edu
Title	Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming
Author	Harvey Jr., Robert
Document Type	Master's Thesis
Keywords	microbes, cyanidiales, Cyanidium, Galdieria, Cyanidioschyzon, Chlorella, algae, stramenophile, fungi, PCR, extremophile, environmental samples, Yellowstone
Abstract	A cultivation-independent approach, sequence analysis of 18S rRNA genes PCR-amplified from environmental DNA, was used to explore the diversity and distribution of eukaryotic microbes inhabiting algal mats in two acidic geothermal streams in Yellowstone National Park. The objectives were to: (1) clarify the identity of mat forming algae in Nymph Creek (2) survey microbial species in the Nymph Creek mat over seasonal intervals along a thermal gradient (3) compare microbial species in the Nymph Creek mat with those in Alluvium Creek mats (4) evaluate microbial species in algal mats formed on different substrates in Alluvium Creek. The results show that a novel red alga dominates high temperature regions (~50ºC) of Nymph Creek and two “Chlorella-like” algae predominate the cooler regions (<38ºC). The predominant algae in Alluvium Creek were distinctly different from those in Nymph Creek. Several stramenophiles and fungi were detected in each algal mat.
Access	Unrestricted
Degree	M.S.
Degree Program	Biological Sciences
Department	Dept. of Biological Sciences
Major Professor	Ferris, Michael; Clancy, Mary
Advisory Committee	Anthony, Nicola
Date Degree Awarded	2009-08-06; *
Format	PDF
URL	See 'reference url' on top or bottom navigation bars
Rights	The University of New Orleans and its agents retain the non-exclusive license to archive and make accessible this dissertation or thesis in whole or in part in all forms of media, now or hereafter known. The author retains all other ownership rights to the copyright of the thesis or dissertation.
Catalog Date	2009-08-14; *
Object File Name	nd000850
Viewer Information	Adobe Reader required for viewing (http://www.adobe.com/products/acrobat/readermain.html)
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Home Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial...

Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming

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